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Customer success is our mission.

Join us at the 63rd ASMS Conference on Mass Spectrometry and Allied Topics and find out more about Waters latest technologies and innovations that enable superior performance in your lab, allowing you to succeed in the science of what’s possible.

Photos from Waters ASMS 2014

Waters MS Users’ Meeting Agenda

Hyatt Regency at the Arch, 315 Chestnut Street, St. Louis, MO 63102

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GENERAL SESSION

Parallel Sessions:

I: TRANSLATIONAL MEDICINE
II: CLINICAL CHEMISTRY & FORENSIC TOXICOLOGY
III: BIOPHARMACEUTICAL
IV: PHARMACEUTICAL
V: FOOD & ENVIRONMENTAL ANALYSIS
VI: CHEMICAL MATERIALS

Saturday, May 30

8:30 AM – 9:30AM

General Session

Registration
Grand Foyer, Fourth Floor

9:30 AM – 10:15 AM

What’s New from Waters

Mike Morris
Senior Director, Mass Spectrometry Research
and
Gordon Kearney
Senior Manager, Product Management, Mass Spectrometry
Waters Corporation, UK

10:15 AM – 11:00 AM

Key Note Presentation 1:
Ambient ms in clinical diagnostics – from microbiology to ms-guided surgery

Professor Zoltan Takats
Professor of Analytical Chemistry
Faculty of Medicine, Department of Surgery & Cancer
Imperial College London
Dr. Zoltan Takats, Reader in Medical Mass Spectrometry, Deputy Head of Section, Section of Computational and Systems Medicine, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London

dr zoltan takatsDr. Takats has obtained his PhD from Eötvös Loránd University, Budapest, Hungary. He has worked as a post-doctoral research associate at Purdue University, Indiana, USA. After returning to Hungary, he served as Director of Cell Screen Research Centre and also as Head of Newborn Screening and Metabolic Diagnostic Laboratory at Semmelweis University, Budapest. Dr. Takats was awarded the Starting Grant by the European Research Council in 2008 and he subsequently become a Junior Research Group Leader at Justus Liebig University, Gießen, Germany. He moved to the United Kingdom in 2012 and currently works as a Reader at Imperial College London. Dr. Takats has pursued pioneering research in mass spectrometry and he is one of the founders of the field of ‘Ambient Mass Spectrometry’. He is the primary inventor of six mass spectrometric ionization techniques and author of 78 peer reviewed publications. He was the recipient of the prestigious Mattauch-Herzog Award of the German Mass Spectrometry Society and the Hungarian Star Award for Outstanding Innovators. He is the founder of Prosolia Inc., Medimass Ltd. and Massprom Ltd., all companies pursuing analytical and medical device development.

11:00 AM – 11:15 AM

Break

Grand Foyer, Fourth Floor

11:15 AM – 12:00 PM

Key Note Presentation 2:
Addressing complex and critical food integrity issues using the latest analytical technologies

The ability to deliver safe, nutritious and authentic food to the world’s growing population is one of the most important and challenging tasks. To cope with this the supply systems of many commodities have become global in nature and are often very complex with multiple players involved. The potential for contamination of foods due to this complexity has increased considerably. When issues arise the ability to identify the cause of the contamination and origin of the food material implicated is often difficult and sometimes impossible.

Of growing concern is the deliberate contamination of foods driven by economic gain. Food fraud has been in existence for many hundreds of years due as global food systems become more complex the opportunities for fraud have greatly increased and the impact these have on consumer health and trust. In 2011 a large study showed that over one third of all fish sold in the US have some sort of fraud associated with it. In Europe in 2013 the red meat supply system was shown to be infiltrated by criminal activity and thus the term food crime emerged.

Food testing is one of the key roles of deterring and detecting food fraud. Due to the multiple types of fraud that can be perpetrated the challenges to be able to identify these are enormous and in some cases still impossible. A review of the types of fraud, the challenges to their detection and the potential for a paradigm shift in detection using rapid evaporative ionization mass spectrometry will be given.

Professor Chris Elliott
Professor of Food Safety and Director of the Institute for Global Food Security
Queen’s University Belfast, Northern Ireland
Chris is currently Professor of Food Safety and Director of the Institute for Global Food Security at Queen’s University Belfast. He has published around 300 peer review articles, many of them relating to the detection and control of agriculture, food and environmental related contaminants. His main research interests are in the development of innovative techniques to provide early warning of toxin threats across the agri- food chains. Protecting the integrity of the food supply chain from fraud is also a key research topic and Chris led the independent review of Britain’s food system following the 2013 horsemeat scandal.

Over the years Chris has developed a high level network of collaborators across Europe, the United States and Asia. He co-ordinates and participates in multiple European framework research projects. He is a founding member of the International School for Advanced Residue Analysis in Food based in France. He is also a visiting Professor at the China Agriculture University in Beijing, a recipient of a Winston Churchill Fellowship and is an elected Fellow of the Royal Society of Chemistry, Society of Biology and the Institute of Food Science and Technology.

12:00 AM – 12:05 PM

Morning Recap

Mike Morris
Senior Director, Mass Spectrometry Research
Waters Corporation, UK

12:05 PM – 1:15 PM

Lunch

Parkview and Grand Ballrooms F&G, Fourth Floor

1:00 PM – 3:00 PM

Face-to-face Technical Service

Waters will have technical service and application specialists available to answer hardware, software and applications questions.
Mills Studio 5, Fourth Floor

PARALLEL SESSION I: TRANSLATIONAL MEDICINE

Location: Grand Ballroom E

Session description:
“Bench-to-bedside” is a phrase that is used in modern translational medicine. The aims of translational medicine is to integrate the sciences of proteomics, genomics, metabolic profiling, transcriptomics and lipidomics for medical studies. The question that researchers face is how can we turn our scientific research findings into real clinical benefits to discover new treatments, improve diagnosis, lower costs and improve patient experiences. Hand in hand with this “bottom-up” approach is the need to integrate clinical findings and observations from epidemiological studies with current molecular “Omics” research. In this workshop we will hear from leaders in the field of proteomics, lipidomics and metabolic profiling for biomedical research. The presentations in this session will cover the topics of lipid analysis in Alzheimer’s research by micro scale LC/MS; MS based molecular imaging for tumor analysis; proteomics platforms for cancer research and an integrated omics platform for medical research support.

1:15 PM – 1:45 PM

Moving towards molecular pathology with desorption electrospray ionization imaging, where we are now and where we’re going

Emrys Jones
Senior Scientist, Imaging Mass Spectrometry, Health Science Business
Waters Corporation, UK

emrysjonesEmrys obtained a 1st class Masters degree in Chemistry from the University of Manchester Institute of Science and Technology (UMIST) and a Ph.D. at the University of Manchester where he developed cluster ion beam approaches for ToF-SIMS imaging of biological samples such as cells and tissue sections.
Postdoctoral positions at both the Wolfson Molecular Imaging Centre (University of Manchester) and the Leiden University Medical Centre (LUMC) in the Netherlands, allowed Emrys to develop a deep understanding of MALDI-MS imaging for a range of applications relating to cancer and neurological conditions. In both positions Emrys focused on enhanced methods of data processing and analysis, multiplexing analyses by combining imaging mass spectrometry with techniques such as positron emission tomography, IHC, ISH and streamlining the connection between analytical chemists and their collaborators in pathology.
Before joining Waters in 2014, Emrys most recently spent two years at Imperial College London in the group of Prof. Zoltan Takats, developing the DESI and REIMS technologies for numerous applications relating to intraoprative mass spectrometry and rapid diagnostics.

1:50 PM – 2:20 PM

Harmonization of ‘Omic Analyses for Translational Medicine Studies – Needs and Realities

M. Arthur Moseley, PhD
Associate Research Professor, Director, Duke Proteomics Core Facility
Duke University, USA

2:20 PM – 2:50 PM

Break

Grand Foyer, Fourth Floor

2:50 PM – 3:20 PM

The role of mass spectrometry for discovering new therapeutics and diagnostic markers

Joseph A. Loo, Ph.D.
Professor, Department of Biological Chemistry, David Geffen School of Medicine
Professor, Department of Chemistry and Biochemistry
UCLA Molecular Biology Institute, University of California-Los Angeles, USA

3:25 PM – 3:55 PM

Metabolomics and lipidomics approaches for biomedical research and biomarker discovery

Giuseppe Astarita
Principal Scientist, Metabolic Phenotyping, Health Sciences
Waters Corporation, USA

4:00 PM – 6:00 PM

Please Join us for a Reception

Beverages and hors d’ oeuvres will be served.
Gateway Terrace, Eighteenth Floor

PARALLEL SESSION II: CLINICAL CHEMISTRY & FORENSIC TOXICOLOGY

Location: Grand Ballroom C
Driving analytical performance in clinical chemistry & forensic toxicology

LC-MS continues to make a valuable contributions to the fields of clinical chemistry and forensic toxicology. As new applications are considered, increasing performance demands are placed upon software, separations and automation tools that further extend the scope of mass spectrometry in laboratories. With this in mind, we present a series of talks describing the efficacy of a broad range of Waters technologies that offer enhanced sensitivity, improved workflows and new perspectives on separations to solve challenges in clinical and forensic laboratories.

1:15 PM – 1:45 PM

The convergence of clinical opportunity and supercritical fluid technology

Matthew Crawford, BSc
Researcher
LabCorp, USA

1:50 PM – 2:20 PM

Targeted, semi-targeted and non-targeted screening approaches using UPLC-QTof-MSE with UNIFI for forensic toxicology analysis

Petur Weihe Dalsgaard, M. Pharm., Ph.D.
Department of Forensic Medicine, University of Copenhagen, Denmark

2:20 PM – 2:50 PM

Break

Grand Foyer, Fourth Floor

2:50 PM – 3:20 PM

A new level of sensitivity and standardization: the promise of integrated micro fluidic devices coupled with mass spectrometry

Paul Rainville
Principal Scientist, Health Sciences Business
Waters Corporation, USA

3:25 PM – 3:55 PM

Successful implementation of online SPE LC-MS/MS in the clinical laboratory

Martijn van Faassen, BSc
PhD Student
Department of Laboratory Medicine, University Medical Center, Groningen, The Netherlands

4:00 PM – 6:00 PM

Please Join Us for a Reception

Beverages and hors d’ oeuvres will be served.
Gateway Terrace, Eighteenth Floor

PARALLEL SESSION III: BIOPHARMACEUTICAL

Location: Grand Ballroom D

Session Description:
The afternoon biopharmaceutical track at the ASMS 2015 Waters User’s meeting will feature technical presentations describing innovations in biotherapeutic characterization techniques that support early molecule development to the monitoring assays that routinely support late development, manufacturing and release. In addition to a Waters presentation detailing highlights of new technologies, tools, and analytical workflows for biotherapeutic analysis, we will have external speakers discussing the use of mass spectrometry for Host Cell Protein (HCP) analysis, Antibody Drug Conjugate (ADC) characterization, and the combined use of Optical and Mass Detection technology for routine monitoring of biopharmaceutical attributes and as a platform for multi-attribute release assays.

1:15 PM – 1:45 PM

Advancements in the characterization of antibody drug conjugates (ADCs) by mass spectrometry

Dr. Alain Van Dorsselaer
Director of BioOrganic Mass Spectrometry Laboratory, Hubert Curien Pluridisciplinary Institute
University of Strasbourg, France

1:50 PM – 2:20 PM

Implementation of Waters RapiFluor-MS Kit and QDa Detector for N-Glycan Analysis in GMP Quality Control Labs

Jeffrey Thompson, PhD
Analytical Specialist I
Regeneron Pharmaceuticals, USA

2:20 PM – 2:50 PM

Break

Grand Foyer, Fourth Floor

2:50 PM – 3:20 PM

Mass Spectrometric Approaches to Biologic Host Cell Protein Identification- A State of the Technology and Perspectives

Matthew Schenauer
Research Scientist II, Biologics Development
Gilead Sciences, USA

3:25 PM – 3:55 PM

Biopharmaceuticals: from the top down, bottom up, outside in, and falling to pieces

Scott J. Berger
Senior Manager, Biopharmaceutical Markets
Waters Corporation, USA

4:00 PM – 6:00 PM

Please Join Us for a Reception

Beverages and hors d’ oeuvres will be served.
Gateway Terrace, Eighteenth Floor

PARALLEL SESSION IV: PHARMACEUTICAL

Location: Grand Ballroom B

Session description:
Today, it’s more important than ever for Pharmaceutical organizations to remain at the forefront of analytical technology adoption. In this session we’ll showcase the pioneering work of scientists around the world working together to use new technology developments in inventive and surprising ways that impact the pharmaceutical lab and have wider implications to the analytical community at large.

1:15 PM – 1:45 PM

New LC/MS techniques for improved sensitivity and selectivity in small molecule ADME analyses

Filip Cuyckens
Scientific Director and Fellow
Janssen R&D, Belguim

1:50 PM – 2:20 PM

Microfluidics for Macromolecules: Leveraging the Ion Key-TQS for Quantitation of Therapeutic Peptides

Lucinda H. Cohen
Director, NJ Discovery Bioanalytics Group
Merck Research Laboratories, USA

2:20 PM – 2:50 PM

Break

Grand Foyer, Fourth Floor

2:50 PM – 3:20 PM

New technologies and the bright future for quantitative bioanalysis

Liz Thomas
Co-founder and CEO
Alderley Analytical, UK

3:25 PM – 3:55 PM

Designing and building the QDa – what it takes to bring a new mass spectrometer to your lab bench

Daniel Kenny
Director, MS Development
Waters Corporation, UK

4:00 PM – 6:00 PM

Please Join Us for a Reception

Beverages and hors d’ oeuvres will be served.
Gateway Terrace, Eighteenth Floor

PARALLEL SESSION V: FOOD & ENVIRONMENTAL ANALYSIS

Location: Grand Ballroom A

Session description:
Advanced MS techniques to ensure quality of life – our food, our environments
The identification of components of interest from food or environmental samples is constantly faced with complex and changeable matrices. In many cases, the compounds of interest are present at much lower levels than matrix components. Whether the task is to ensure ingredient safety and consistency, to screen products for known contaminants or residues, to identify new potential contaminants or to search for markers of authenticity or food fraud, the technologies that improve confidence in the analytical result are key to the success of today’s laboratories.
In this session, four keynote speakers will address our audience on how Waters’ novel technologies are helping to ensure the quality of the food we eat and the environment we live in. Novel technologies such as APGC, ion mobility combined with high resolution MS, IonKey and the informatics platforms that enable the collection and interpretation of multi-faceted datasets from such advanced analytical instrumentation.

1:15 PM – 1:45 PM

A train full of ion mobility benefits for food analysis, arriving at the next platform

Current trends indicate that more than 500 compounds are routinely used under strict regulation on a global basis. With increasing global trade, there is a requirement for multi-analyte screening strategies capable of efficiently detecting residue violations to protect consumer safety. Benefits of full spectra acquisition and the specificity of accurate mass measurement is well characterised and is used in combination with, time tolerances, isotope fits, fragment ions/ratios and response thresholds to reduce false positive/negative identifications in screening assays. Nonetheless, it is a challenge to identify targeted compounds present in the sample with a large number of co-extracted matrix components, in a time efficient manner.
Confidence in the use of CCS as an identification parameter, will be shown from the initial international feasibility studies. Day to day, month to month, injection to injection and 25 matrix to matrix comparisons will be presented, where CCS RSD’s of < 1% have been achieved. The application of ion mobility to remove false positive identifications and importantly false negative identifications, will be shown. In addition CCS can provide further confidence where only monoisotopic information is available at low level detection. Unique pesticide protomer identification has been discovered. Also the power of CCS selectivity is illustrated when comparing different chromatographic profiles, CCS can provide a route to flexibility in chromatographic methodology employe. The extensive study using ion mobility has been performed using routine ion mobility screening platforms.
Dr Severine Goscinny
Scientific Researcher
Department of Food, Medicines and Consumer Safety,
Scientific Institute of Public Health, Belgium

1:50 PM – 2:20 PM

Using ‘small molecule’ CCS from ion mobility mass spectrometry for ID and prediction

Enhancements in both hardware and software technology have resulted in an increase in the use of ion mobility as a routine analytical tool. Workflows have been developed where collision cross section (CCS) has been used as an additional identification parameter. The current approach to develop a CCS screening library to be used in non targeted screening assays requires collision cross section measurements to be performed using high purity standards and the determined CCS value entered into the library.
Accurate measurement and better prediction of collision cross sections (CCS) through molecular modelling opens up powerful new opportunities including providing precursor and product ion CCS databases that can confirm structures and substructures, assigning positional isomers without requiring reference materials for comparison and optimising ion mobility separations in silico. This talk highlights unique opportunities in food and environmental applications including rapidly generating theoretical CCS and utilising them for identification and confirmation.
Dr Cris Lapthorn
Researcher
University of Greenwich, UK
Cris Lapthorn previously held positions as Head of Open-Access Mass Spectrometry at Pfizer and as a mass spectrometry specialist at Novartis, Horsham. He is now Head of Mass Spectrometry Services at the University of Greenwich.
He a recipient of the 2014 British Mass Spectrometry Society (BMSS) Bordoli Prize and co-authored an ion mobility mass spectrometry review that is one of the top 10 most accessed publications in the highest impact factor mass spectrometry journal, Mass Spectrometry Reviews. He has been invited to tutor on courses including the BMSS Ion Mobility Special Interest Group meeting.
His research predominantly utilises ion mobility mass spectrometry, often in combination with molecular modelling. Ion mobility can separate ions based on their drift-time through a buffer gas, so can give information about shape, size and charge distribution and provide separation in milliseconds in addition to mass spectrometry data.

2:20 PM – 2:50 PM

Break

Grand Foyer, Fourth Floor

2:50 p.m. – 3:20 p.m.

Accurate mass screening for known and unknown nitrogenous economic adulterants with workflows in UNIFI

Economic adulteration of milk involves adulterating milk with nitrogen rich compounds to bias the widely used Kjeldahl protein assay. This issue garnered global attention during the melamine scandal in China which resulted in the sickening and deaths of infants. Today a heightened awareness of the melamine issue, availability of reliable analytical test methods, and recently introduced regulations, collectively decrease the risk of repeat incidence of the melamine crisis. However, the motivation for economic adulteration remains. Therefore it is conceivable for the emergence of alternative nitrogenous compounds. Hence, continuous development of new analytical screening methodologies capable of detecting the presence of externally introduced highly nitrogenous compounds is needed to ensure food safety of milk based ingredients and finished products.
Triple quad based LC-MS platforms are widely used for the analysis of melamine. The use of triple quad, though sensitive and specific, requires a priori knowledge of target analytes. However the use of accurate mass based QTOF platform allows for the simultaneous analysis of targeted and non targeted compounds. A high resolution accurate mass screening method was developed using a QTOF platform and HILIC separation. The method employs a simple dilution based sample preparation and stable isotope labeled internal standards to enable the analysis of economic adulterants present in milk derived ingredients. A number of nitrogenous economic adulterants were targeted for rapid analysis. Further, a number of additional nitrogenous compounds were screened to evaluate method’s applicability beyond the target analytes.
Dr Nicholas Cellar
Research Scientist, Analytical Research and Services
Abbott Nutrition, USA
Dr. Cellar is a Research Scientist at Abbott Nutrition and has spent six years in industry as an analytical chemist. He specializes in separations and mass spectrometry, and has developed and validated a number of methods for food safety applications as well as vitamin determination. In his current role, Dr. Cellar manages The Food Safety Laboratory and Raw Materials Laboratory within R&D in Abbott Nutrition.
Prior to Abbott Nutrition, Dr. Cellar worked in the Analytical Sciences department at the Dow Chemical Company. At Dow, he supported R&D efforts by developing methods for a diverse range of chemicals in a variety of matrixes.
Dr. Cellar received his doctorate in Analytical Chemistry from the University of Michigan and his Bachelors of Science from Miami University. His graduate research involved development of microfluidic devices for the sampling, separation, and detection of amino acid neurotransmitters. He has a number of publications and has presented at professional conferences around the world.

3:25 p.m. – 3:55 p.m.

Advanced technologies for analysis of poly-halogenated dioxins and furans in controlled burn samples

Dr Frank Dorman
Associate Professor, Biochemistry and Molecular Biology
Eberly College of Science, Penn State University, USA
Dr Frank Dorman graduated with a PhD in Analytical Chemistry from the University of Vermont in 1993 when he joined what was then called Inchcape Testing Services which was subsequently acquired and renamed to become part of the InterTek network. In 1996 he joined Restek and rose quickly to the position of Director of Technical Development, a position he held for 10 years, before taking his current position at The Pennsylvania State University. He is currently an Associate Professor at in the Department of Biochemistry and Molecular Biology and a member of the Forensics Science Program at the Eberly College of Science. In addition to undergraduate and graduate student instruction, he continues to pursue research interests in gas and liquid chromatography and their application to trace analysis in complex matrices.

4:00 PM – 6:00 PM

Please Join Us for a Reception

Beverages and hors d’ oeuvres will be served.
Gateway Terrace, Eighteenth Floor

PARALLEL SESSION VI: CHEMICAL MATERIALS

Location: Grand Ballroom H

Session description:
This session will focus on the application of a number of novel technologies that enable scientists to better characterize complex chemical materials such as petroleum, next generation biofuels and polymers. In addition to sample complexity the chemical diversity of samples presents additional challenges for mass spectrometry based characterization. The speakers will present developments in source and inlet technologies in combination with mass spectrometry to address these challenges.

1:15 PM – 1:45 PM

UPC2-MS – another new kid on the block or the missing link?

The false dawns and promises of SFC have come and gone, now at last robust instrumentation has been delivered. UPC2-MS is a platform that comfortably sits alongside LC-MS and GC-MS affording extended capability and complementarity for the analyst. At Southampton Chemistry UPC2-MS delivers unique solutions across a broad range of application areas, e.g. synthetic organics, petrochemistry, lipids, nucleotides and many other areas .
This technology has been incorporated in to the open access facility in Chemistry and provides new approaches as well as complementing existing systems. The breadth of chemistries, column phases and different ionization approaches will be used as examples of the positive impact that UPC2-MS has and is making.
Dr. G. John Langley
Associate Professor of Mass Spectrometry
University of Southampton, UK

1:50 PM – 2:20 PM

Advancing atmospheric pressure ionization though GC-AP/MS, ASAP, and matrix-assisted ionization

Horning’s group in the 1970’s developed atmospheric pressure ionization (API) mass spectrometry (MS), but it was electrospray ionization that drove the commercialization of API-MS as a liquid introduction technology. The utility of gaseous introduction of volatile compounds into an API source and new developments in GC-API/MS and ASAP-MS will be discussed along with the newly discovered matrix-assisted ionization technology that allows ionization of low and high mass compounds on API instruments without use of a laser, high voltage, or even heat.
Charles N. McEwen
Houghton Professor of Chemistry and Biochemistry at University of the Sciences
University of the Sciences, USA

2:20 PM – 2:50 PM

Break

Grand Foyer, Fourth Floor

2:50 PM – 3:20 PM

APGC and other MS techniques in Fuels research

Dr. Vladislav V. Lobodin
Research Faculty, National High Magnetic Field Laboratory
Future Fuels Institute, Florida State University, USA

3:25 PM – 3:55 PM

Harnessing the power of CCS for routine screening applications on the UNIFI informatics platform

Professor Cristina Nerin
Professor of Analytical Chemistry
University of Zaragoza, Spain

4:00 PM – 6:00 PM

Please Join Us for a Reception

Beverages and hors d’ oeuvres will be served.
Gateway Terrace, Eighteenth Floor

Each of the afternoon parallel sessions will follow the same schedule, with a 5 minute Q&A period and a 5 minute transition period at the end of each 20 minute presentation. Participants wishing to attend a presentation in a different parallel session are welcome to do so.

Join us for breakfast at the following technical seminars.

Pre-registration is required.

Monday, June 1

7:00am – 8:00am

What’s New From Waters

Presenters: Emma Marsden-Edwards, MS Product Manager, and Nick Tomczyk, Principal Analytical Scientist, Waters Corporation

Location: Renaissance St. Louis Grand, 800 Washington Ave, St. Louis, MO 63101 | Majestic E Ballroom, second floor

Join us for breakfast and be one of the first to view the latest advances in mass spectrometry from Waters. You will also have the opportunity to meet some of our MS research experts, and preview the scientific data we will be presenting at this year’s conference.

Space is limited, pre-registration is required.

Pre-Register

Monday, June 1

7:00am – 8:00am

Quoi de neuf avec Waters – This is a special presentation in French for French speaking ASMS attendees

Location: America’s Center Convention Complex, 701 Convention Plaza, St. Louis, MO 63101 | Room 231

7:00am – 7:10am – Welcome : David Lascoux
7:10 – 7:30am – Les apports de la mobilité ionique couplée à la spectrométrie de masse : Alain Van Dorsselaer
7:30am – 7:45am – Nouvelle plate forme d’imagerie Waters : Emmanuelle Claude
7:45am – 8:00am – Encore plus de mobilité ionique : David Lascoux

We hope French speaking attendees can join us for breakfast to kick off ASMS!

Space is limited, pre-registration is required.

Pre-Register

Monday, June 1

7:00am – 8:00am

Transforming Sample Preparation

Location: America’s Center Convention Complex, 701 Convention Plaza, St. Louis, MO 63101 | Room 260/267

Presenter: Xin Zhang, Senior Research Chemist, Waters Corporation

Much of the focus in liquid chromatography (LC) during the last decade has been on improving the speed and resolution of the separation. An example of that was in 2004 when the landscape of LC changed with the introduction of the ACQUITY UPLC system, the first holistically-designed chromatographic system to take full advantage of sub-2-µm particle columns. One consequence of improving and/or shortening chromatographic analysis times is that the laboratory throughput bottleneck has now moved from analysis time to sample preparation time. As an industry leader in sample preparation products and methodologies, Waters understands these challenges and has been focusing on simplifying and streamlining sample preparation. To address this need we introduce a new, novel sorbent for small molecule sample preparation that produces cleaner samples in less time and with fewer steps.

In small-molecule analyses, complex sample matrices, price pressures, and stringent analytical method requirements place additional demands on separation scientists. Using “good enough” sample preparation approaches that fail to adequately prepare samples can lead to shortened column lifetimes and poor method sensitivity and precision due to ion suppression or enhancement. To overcome these issues, Waters has developed a new, easy-to-use, streamlined approach that is based on our water-wettable, reversed-phase sorbent Oasis. This new and exciting product produces high recoveries, improved sample flow capabilities, and reduced matrix effects in far less time than typical solid-phase extraction protocols. In our presentation we will demonstrate how this proprietary, patent-pending sorbent addresses the needs of today’s analytical scientists by producing cleaner samples in not only less time but within a predictable time frame due to lack of plugging. For those conscious of environmental concerns and/or the cost of solvent waste disposal, solvent consumption can be reduced by almost a factor of two.

We hope you can attend!

Space is limited, pre-registration is required.

Pre-Register

Dr. Xin Zhang is currently a senior research chemist at Waters Corporation. She received her Ph. D degree in analytical chemistry from University at Buffalo, The State University of New York in 2007. At Waters Corporation, she has worked in Consumables R&D and the Technology Advancement Department. She has been focused on evaluating different sample preparation techniques such as solid phase extraction(SPE), protein precipitation(PPT), solid supported liquid extraction (SSLE) as well as the Ostro Pass-through sample prepare technique. Recently, she has been working on simplified SPE procedures for the Oasis family. In addition, she also helps customers understand sample preparation by delivering educational seminars and trouble shooting. She can be contacted at xin_zhang@waters.com

Monday, June 1

7:00am – 8:00am

Microflow LC-MS providing enhanced sensitivity from research to routine applications

Presenter: TBD

Location: Jim Murphy, Principal Consulting Chemist, Core Research, Waters Corporation

The significant benefits of microflow LC-MS must also be accompanied by ease-of-use, robustness and a capability to withstand the rigours of an analytical method. This presentation will introduce you to Waters ionKey/MS system and review the versatility for DMPK applications. Please join us for breakfast to learn more about this cutting edge technology.

We hope you can attend!

Space is limited, pre-registration is required.

Pre-Register

Tuesday, June 2

7:00am – 8:00am

Translational Medicine breakfast presentation

Location: Renaissance St. Louis Grand, 800 Washington Ave, St. Louis, MO 63101 | Majestic E Ballroom, second floor

Presenter: Professor Zoltan Takats, Professor of Analytical Chemistry, Faculty of Medicine, Department of Surgery & Cancer, Imperial College London

Additional details will be posted shortly.

Join us for this exclusive breakfast presentation.

Space is limited, pre-registration is required.

Pre-Register

Tuesday, June 2

7:00am – 8:00am

The transformation of ion mobility and CCS technology into a core decision making tool

Presenters: Dr Cris Lapthorn, Researcher, University of Greenwich, and Russell Mortishire-Smith, Senior Consultant Scientist, Pharmaceutical Business, Waters Corporation

Location: America’s Center Convention Complex, 701 Convention Plaza, St. Louis, MO 63101 | Rooms 260/267

In this breakfast meeting we will host a forum for sharing exciting new work in the field of ion mobility. Cris Lapthorn will present his and his peers work on cutting edge approaches that could transform the use of ion mobility and fully leverage the power of the next generation of core analytical instrumentation. Cris will discuss making better decisions based on benchmarking and improving experimental and theoretical calculations. Finally, Russell Mortishire-Smith will join Cris and discuss areas where IMS and CCS could be deployed in the future.

We hope you can join us!

Space is limited, pre-registration is required.

Pre-Register

Wednesday, June 3

7:00am – 8:00am

Bringing Glycan Analysis from the Dark Ages to a New Age of Enlightenment – A discussion of disruptive LCMS technologies and workflows for glycan analysis.

Presenter: Professor Pauline M. Rudd, Principal Investigator, National Institute of Bioprocessing Research and Technology (NIBRT), Dublin, Ireland

Location: Renaissance St. Louis Grand, 800 Washington Ave, St. Louis, MO 63101 | Majestic E Ballroom, second floor

This breakfast seminar will detail the remarkable progress that has recently been achieved in advancing workflows for glycan sample preparation, separations, and analysis by mass spectrometry. Professor Rudd will enlighten us with a review of glycan structure and functionality, and survey the analytical workflows her laboratory has developed for glycan structural characterization and profiling. Her pioneering adoption of UPLC for glycan separations, and development of Glucose Unit based normalized retention methods and informatics has delivered higher quality quantitative glycan profiles with superior method robustness and higher confidence glycan assignments.

During this session you will also hear from Waters about RapiFluor-MS, a disruptive new glycan labeling technology that enables researchers to go from intact glycoprotein to a ready to analyze sample within 30 min, to obtain greater sensitivity for FLR quantification, and realize 100-1000x greater MS response versus other traditional and instant labeling technologies. This revolution in labeling technology has not only improved the ability to obtain comprehensive characterization of glycan mixtures, but combines with a equally disruptive technology, the ACQUITY QDa Mass Detector, to usher in a new era of routine glycan profile monitoring with the additional confidence and capabilities imparted by mass detection.

Please register early, as spaces will be limited for this exciting ASMS breakfast seminar.

Pre-Register

pauline ruddProfessor Pauline M. Rudd BSc, LRIC, MA (Oxon), PhD is the Research Professor of Glycobiology at University College, Dublin. She also heads the GlycoSciences Research Group at (NIBRT), the National Institute for BioProcessing Research and Training in Ireland. Her laboratory has extensive experience with the analysis of therapeutic glycoproteins, and produced significant contributions to questions of glycan structure and function and the emerging field of glycomics based biomarker discovery.

Professor Rudd obtained her BSc in Chemistry at the University of London and a PhD in Glycobiology at the Open University, UK. She was a Founding Scientist of Wessex Biochemicals (later Sigma London), and has served as aVisiting Research Associate at The Scripps Research Institute, CA, Visiting Professor of Biochemistry at Shanghai Medical University PRC, Visiting Scientist at Ben Gurion University of the Negev, Israel and Erskine Visiting Fellow, Canterbury University, Christchurch, New Zealand. She is a Fellow of the Royal Society of Medicine, a Visiting Investigator at AStar in Singapore and a Visiting Professor at St. George’s Hospital in London and an Adjunct Professor at Northeastern University, Boston, NUI Galway and Trinity College Dublin. She has more than 300 scientific publications and has given over 350 lectures and seminars at international meetings. In 2010 she was awarded the James Gregory Medal andin 2012 her NIBRT laboratory was recognized as a Waters Center of Innovation for Glycobiology and Glycan Analysis.

Wednesday, June 3

7:00am – 8:00am

Progenesis QI: Your Gateway to Reliable Results. The latest on software developments and applications.

Location: America’s Center Convention Complex, 701 Convention Plaza, St. Louis, MO 63101 | Room 230

Join us for three exciting presentations. Start your day with these engaging topics.

  • Introduction and ASMS highlights from Waters and Progenesis QI
    Presenter: Robert Tonge, Senior Product Manager OMICS Informatics, Waters Corporation, Manchester, UK
  • Progenesis QI for proteomics: a first-rate solution for relative and absolute quantitative workflows
    Presenter: Richard Remko Sprenger, Senior Research Scientist and Laboratory Manager, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark
  • Progenesis QI: Introducing an extra gear for complex data set handling in metabolomics
    Presenter: Geert Goeminne, VIB Department of Plant Systems Biology, University of Gent, Gent, Belgium

Progenesis QI software enables you to Quantify and then Identify compounds that are significantly changing in your samples. With support for all common vendor data formats and a highly intuitive, visually guided workflow, Progenesis QI software helps you to rapidly, objectively and reliably discover compounds of interest using multi-group experimental designs, with the capability to handle the large sample sets typical of today’s experiments. As well as conventional data-dependent analysis (DDA), Progenesis QI supports Waters MSE and HDMSE data independent analysis (DIA). Uniquely, the software also takes advantage of the additional dimension of resolution offered by ion mobility separations to give improvements in the accuracy and precision of compound identification and quantification. In this Breakfast Seminar, we will introduce you to the workflows and concepts behind Progenesis QI and highlight the new features in the product. We will then showcase the software with examples of small and large molecule analyses.

Metabolic profiling of plants involves the processing of huge complex data files containing thousand of mass spectral peaks. As the plant kingdom contains hundred thousands of small molecules of which only a very small percentage is identified up to now, it is still an elaborative task to interpret UPLC-HRMS data derived from any untargeted plant metabolomics study. In our lab most cases of metabolic profiling setups consists of the comparison between a set of normal plants and a set of mutant plants or often several allelic lines for a particular mutation, including biological repeats enabling advanced statistics. Elucidating the biological mechanisms that are affected by the introduction of a mutation is the final aim of the whole process. Therefore solid statistics and accurate compound identification strategies are crucial, but also the quality of basic chromatogram alignment and peak picking processes have a huge impact on the quality and the accuracy of the biological interpretation. Here we present a high throughput method for the metabolic profiling data processing of two mutant Maize lines compared to their wild type through Progenesis QI v 2.0. The aim of this study was to reveal the effect of the mutation on lignification processes in Maize with the focus on improving second generation crops for the production of bioethanol.

We hope you can attend!

Space is limited. Pre-registration is required.

Pre-Register
Robert Tonge
Robert Tonge is Senior Product Manager for OMICS Informatics at Waters Corporation, Manchester UK.
Robert has been at Waters for 4 years in both Product Development and Field Marketing and has approaching 20 years experience in OMICS science.
He trained initially in Biochemistry and Toxicology before moving to ‘Big Pharma’ where he worked in Discovery and Translational Research across a wide variety of disease areas from cancer, cardiovascular disease and infection research.

Richard Sprenger
Richard Sprenger is a cell biologist, proteomics expert and avid musician. He has a M.Sc in Medical Biology and a PhD in Medical Biochemistry from the University of Amsterdam, where he developed and applied novel quantitative LC-MS technologies to study vascular disease and metabolic disorders, and co-founded the Clinical Proteomics Facility at the Academic Medical Center (AMC) in Amsterdam. Afterwards he focused on refining all aspects of the proteomics toolbox at the Protein Research Group at the University of Southern Denmark (SDU) in Odense. He has published and co-authored over 20 papers in a wide variety of journals including JPR, MCP, JBC, as well as Chemistry & Biology, Blood and the ISME Journal. He recently joined the Lipid Research Group at SDU and established a new niche, proteolipidomics, combining state-of-the-art absolute quantitative lipidomics and proteomics approaches to uncover the regulatory circuitries of lipid metabolism.

Geert Goeminne
• Bachelor Degree in Biochemistry
• Master Degree in Biotechnology and Biochemistry
• Started working at the department of Plant Systems Biology at VIB (Vlaams Instituut voor Biotechnologie/ Flemish Institute for Biotechnology), Ghent University, Technology Park Ghent, Belgium in 2000 until current
• Specialized in metabolomics and instrumentation such as HPLC, UPLC and low and high resolution LC-MS
• Focusing on generation of a high throughput metabolomics pipeline
• Belgian Nationality
• Born on 4th May 1976
• 28 co-author publications (1 Science paper)

Practical Considerations for LC/MS Bioanalysis of Proteins via the Surrogate Peptide Approach

Presenter: Dr. Erin Chambers, Waters Corporation

Location: America’s Center Convention Complex, 701 Convention Plaza, St. Louis, MO 63101 | Room 231

Proteins and peptides represent a growing class of therapeutics due to their target specificity, lower toxicity and higher potency. Historically, these compounds have been quantified using ligand binding assays (LBAs). Recently, there has been a growing trend toward the use of LC/MS which offers the benefits of multiplexing, improved specificity, broader linear dynamic range and faster method development times. In addition, LC/MS avoids common LBA shortcomings such as cross-reactivity and anti-drug antibody effects. However, one challenge associated with quantification of proteins by LC/MS is the lack of a single standardized workflow, and the multitude of options within a workflow that make optimization difficult and time-consuming. This workshop aims to provide practical method development guidance and comparative data for the choice of surrogate peptide, protein-level pre-fractionation, pellet digestion, peptide-level clean-up, internal standard (IS) selection, and digestion conditions. Data for infliximab, bevacizumab, and trastuzumab will be presented.

We hope you can attend!

Space is limited. Pre-registration is required.

Pre-Register

Attend ASMS 2015 and learn about Waters’ exciting new launches, as well as our innovative technologies, including:

desi system

Full Spectrum Molecular Imaging

combines advanced MS imaging technologies to provide comprehensive molecular distribution information from a variety of sample types.


ionKey/MS

gives you the power to analyze smaller samples, do more when you have limited samples, and save time and money.


qda

ACQUITY QDa Detector

is the industry’s most accessible, affordable and usable mass detector. It is as intuitive as an optical detector with the robustness to handle all of your analyses.

Tandem Quadrupole MS/MS

Xevo TQ-S, Xevo TQ-S micro, and Xevo TQD are the analytical tools of choice for all of your quantitative UPLC-MS/MS applications, delivering the ultimate in versatility for a wide variety of applications – both quantitative and qualitative.

Quadrupole Time-of–Flight MS/MS

Xevo G2-XS QTof incorporates the XS Collision Cell and StepWave ion optics for unsurpassed levels of robust sensitivity.

T-Wave Ion Mobility QTof MS

SYNAPT G2-Si gives you access to the highest levels of information and make scientific discoveries not possible with high mass resolution alone.

GC/MS

The APGC source enables fast switchover between LC-MS and GC-MS, utilizing soft ionization to deliver high sensitivity spectra, which are rich in molecular ion information.

Universal Ion Sources

enable the widest range of ionization techniques to be utilized universally across a single analytical platform.


Meeting Dates:
May 30 – June 4, 2015

Waters Hospitality Suite:

June 1 – 3
8:00pm – 11:00pm

Renaissance St. Louis Grand
800 Washington Avenue
St. Louis, MO 63101
Majestic E Ballroom, second floor

Booth #160
Exhibit Halls 1 – 4

Exhibit Hours:
Sunday, May 31
7:45pm – 9:00pm
Monday, June 1
9:00am – 5:00pm
Tuesday, June 2
9:00am – 5:00pm
Wednesday, June 3
9:00am – 5:00pm
Thursday, June 4
9:00am – 2:30pm

America’s Center Convention Complex
701 Convention Plaza
St. Louis, MO 63101
Get Directions