Complete sample preparation for released N-Glycans in
30 minutes with 3 simple steps.

Start with your sample

Glycoprotein

Intact mAb Mass Check Standard

glycoprotein sample

Step 1

< 15 min

Released N-Glycans by Deglycosylation

GlycoWorks™ Rapid PNGase F and Buffer, and RapiGest™ SF Surfactant

released n-glycans by deglycosylation

Step 2

5 min

Labeled Glycans

GlycoWorks RapiFluor-MS™ Labeling

Step 3

10 min

Purified Glycans

GlycoWorks HILIC µElution Plate or Cartridges

labeled glycans

Featured Application Notes

Rapid Preparation of Released N-Glycans for HILIC Analysis Using a Novel Fluorescence and MS-Active Labeling Reagent

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Exploiting RapiFluor-MS Labeling to Monitor Diverse N-Glycan Structures via Fluorescence and Mass Detection

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New Capabilities for Monitoring Released N-Glycans through the Combined Use of RapiFluor-MS Labeling, ACQUITY UPLC H-Class Bio System, and Serial Fluorescence/QDa Mass Detectiont

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Transferring RapiFluor-MS Labeled N-Glycan HILIC Separations Between UPLC and HPLC

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Introducing the GlycoWorks RapiFluor N-Glycan Analysis Kit

glycan analysis kit
Product Information
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Here are the facts.

0x

Faster than Traditional Labeling Techniques

30 min

RapiFluor-MS

4 hrs

Instant Labeling
Techniques

8 hrs

Traditional Labeling
Techniques

0x

Greater Fluorescence Signal than Traditional Labeling Techniques

RapiFluor-MS 100%
Instant AB 52%
2-AB 7%
Procainamide 7%

Percentage of Relative Performance

0x

Increase in MS Signal Response versus Instant Labeling Techniques

RapiFluor-MS 100%
Instant AB < 1%
2-AB ~ 1%
Procainamide 30%

Percentage of Relative Performance

A Smart Workflow with No Compromises

By combining rapid deglycosylation with fast labeling, sample preparation of N-glycans can be completed quickly and efficiently, so you can move on to analysis.

Join Waters in thinking outside the box for released N-glycan analysis

n-glycan analysis - speed throughput

Speed/Throughput

flr quantification

FLR Quantification

n-glycan analysis ms performance

MS Performance

Bringing MS into Routine Monitoring with the ACQUITY QDa Detector

acquity qda detector
  • Greater confidence with mass confirmation of FLR peaks
  • Faster analysis with targeted MS profiling
  • Easily integrated with GMP-compliant workflows with Empower 3 Software

RapiFluor-MS labeled N-glycans monitored using an ACQUITY UPLC H-Class Bio System with detection by FLR and the ACQUITY QDa Detector, for confident assignment of known structures and mass data for unassigned structures.

Would you like mass information for your fluorescence peaks?

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The Next Level of Characterization with Xevo G2-XS QTof MS

xevo QTof MS
  • Easily and confidently assign low-level glycan components
  • Achieve unparalleled levels of MS and MS/MS performance by combining best-in-class technologies

Comparison of LC-MS chromatogram of 2AB-labeled N-glycans versus RapiFluor-MS-labeled N-glycans from the same mAb. 100 fold greater ESI+ MS response was observed for RapiFluor-MS-labeled sample. An MS spectrum (insert) shows the summed spectrum for a low abundance glycan within the sample. 

Let us show you how.

Onsite, lunch-time seminar and “show and tell”

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Waters Glycan Analysis Solutions

Intact Protein Analysis

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intact protein analysis
PRODUCT INFO

Subunit
Analysis

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glycan subunit analysis
PRODUCT INFO

Glycopeptide Mapping

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glycan glycopeptide mapping
PRODUCT INFO

Released N-Glycan Analysis

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released n-glycan analysis
PRODUCT INFO

Monosaccharide
Composition

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glycan monosaccharide composition
PRODUCT INFO