A Simple Cleanup Protocol Using a Novel SPE Device for UPLC-MS/MS

see our special offer

 In this experiment, a novel solid phase extraction (SPE) device, the Oasis PRiME HLB Cartridge, was used in the sample preparation of milk samples as a cleanup method for multi-residue veterinary drug analysis. The initial extraction and protein precipitation was done by adding acidified acetonitrile. The extract was cleaned up by pass-thru SPE using the Oasis PRiME HLB Cartridge prior to UPLC-MS/MS analysis. Sample extraction, chromatographic and mass parameters were all optimized.

As a result, within the ranges of 0.1 to 10.0 μg/mL spiking concentrations, 9 classes of 72 veterinary drugs including sulfonamides, fluoroquinolones, β-agonists, macrolides, glucocorticoids, amphenicols, β-lactams, cephalosporins, penicillin, and tetracyclines, the percent recoveries are all within 50% to 130%, and the RSD <20% (n=5). This method is simple, rapid, and accurate, suitable for multi-residue veterinary drug analysis of milk.

Receive 20% off consumable products featured in this
Application Note of the Month

Use offer code W172SIMPLE at checkout. 

Expires May 31, 2017

Share this offer with a colleague

Receive 15% off Waters 96-Well Collection Plates

Use offer code W17296WELL

Expires: May 31, 2017

*This offer applies to online purchases only through the Waters Order Center. Only valid in the USA and Europe. Promotion applies to orders of up to $5,000 dollars. Standing orders and order of custom products are excluded. Not available to distributors or companies that resell chromatography products and supplies. Void where prohibited or in countries where online shopping is not available.
Subject to change at any time.